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啤酒酵母AS2.1416海藻糖合成酶基因tps1的cDNA克隆及序列分析
题名：生物工程学报
作者：董志扬段瑜侃陈红漫金城张树政
出版年：2000
卷期:03
页码:
分类:总论
内容摘要: The trehalose 6 phosphate synthase gene tps 1 was amplified from yeast S.cerevisiae AS2.1416 cDNA library by polymerase chain reaction.This 1.5 kb fragment was cloned into Pst I and Bam HI sites of pGEM T easy vector and the sequence of the gene indicated the cloned tps 1 gene contained 1485 nucleotides encoding for 495 amino acid and shared a sequence homology of 99.6% with that from S.cerevisiae S288C.
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