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枯草芽孢杆菌α-乙酰乳酸脱羧酶基因在啤酒酵母工业菌株中的表达
题名：微生物学报
作者：郭文洁何秀萍铁翠娟张博润
出版年：2001
卷期:01
页码:
分类:总论
内容摘要: Yeast YIp\type expression recombinant plasmid（pCMA2\1） was constructed. The expression of α\acetolactate decarboxylase gene from \%Bacillus subtilis\% was controled by \%CUP1\% promoter and its own terminator. The recombinant plasmid pCMA2\1 was introduced into the brewer‘s yeast PJ3\5. Transformants were selected using copper resistance as selected marker. The results of activity assay showed that PJ3\5 didn‘t produce α\acetolactate decarboxylase（ALDC） where as the activity of α\ALDC in transf
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